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Title: Improving microarray spots segmentation by k-means driven adaptive image restoration
Authors: Δασκαλάκης, Αντώνης
Κάβουρας, Διονύσης Α.
Μπουγιούκος, Παναγιώτης
Κωστόπουλος, Σπυρίδων
Αργυρόπουλος, Χρήστος
Contributors: Νικηφορίδης, Γεώργιος Σ.
Item type: Conference publication
Conference Item Type: Poster
Keywords: Microarray;Gene;Μικροσυστοιχίες;Γονίδιο
Subjects: Technology
Biomedical engineering
Βιοϊατρική τεχνολογία
Issue Date: 14-May-2015
Date of availability: 14-May-2015
Abstract: Complementary DNA microarray experiments are used to study human genome. However, microarray images are corrupted by spatially inhomogeneous noise that deteriorates image and consequently gene expression. An adaptive microarray image restoration technique is developed by suitably combining unsupervised clustering with the restoration filters for boosting the performance of microarray spots segmentation and for improving the accuracy of subsequent gene expression. Microarray images comprised a publicly available dataset of seven images, obtained from the database of the MicroArray Genome Imaging & Clustering Tool website. Each image contained 6400 spots investigating the diauxic shift of Saccharomyces cerevisiae. The adaptive microarray image restoration technique combined 1/a griding algorithm for locating individual cell images, 2/a clustering algorithm, for assessing local noise from the spot’s background, and 3/a wiener restoration filter, for enhancing individual spots. The effect of the proposed technique quantified using a well-known boundary detection algorithm (Gradient Vector Flow snake) and the information theoretic metric of Jeffrey’s divergence. The proposed technique increased the Jeffrey’s metric from 0.0194 bits to 0.0314 bits, while boosted the performance of the employed boundary detection algorithm. Application of the proposed technique on cDNA microarray images resulted in noise suppression and facilitated spot edge detection.
Description: Proceedings
Language: English
Citation: Daskalakis, A., Cavouras, D., Bougioukos, P., Kostopoulos, S., Argyropoulos, C., et al. (2006). Improving microarray spots segmentation by k-means driven adaptive image restoration. Proceedings of the International Special Topic Conference on Information Technology in Biomedicine. Ioannina, Greece, 26th-28th October 2006. Available from:
Conference: International Special Topic Conference on Information Technology in Biomedicine
Access scheme: Publicly accessible
License: Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες
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