Please use this identifier to cite or link to this item: http://hdl.handle.net/11400/4995
Title: Taguchi experimental design for medium optimization for enhanced protease production by Bacillus subtilis HB04
Authors: Venil, C.K.
Lakshmanaperumalsamy, P.
Item type: Conference publication
Keywords: Bacillus subtilis HB04;Protease;Taguchi design;Contribution;Πρωτεάση;Συνεισφορά;Taguchi σχεδιασμός
Subjects: Technology
Chemical technology
Τεχνολογία
Χημική τεχνολογία
Issue Date: 29-Jan-2015
2009
Date of availability: 29-Jan-2015
Publisher: Νερατζής, Ηλίας
Σιανούδης, Ιωάννης
Abstract: Bacillus sp. is specific producers of proteases amongst bacteria and protease enzymes are of significant ones due to their multifarious applications. In this research,Taguchi experimental design was applied to optimize the conditions for protease production by Bacillus subtilis HB04. This approach facilitated the study of interaction of a large number of variables spanned by factors and their settings with a small number of experimental runs leading to considerable economy in time and cost for the process optimization. The objective of this research was to determine the significant parameters in the production of protease by Bacillus subtilis HB04 in submerged fermentation. Five factors viz., carbon, nitrogen, metal ions, temperature and agitation, each at four levels were selected and an orthogonal array layout of L16 (45) performed. The experiment result indicated that dextrose (1 %), (NH4)2PO4 (0.5 %), temperature (30oC) and agitation (100 rpm) were the important factors for protease production by Bacillus subtilis HB04 in submerged fermentation at the optimum levels.
Language: English
Citation: Venil, C.K. and Lakshmanaperumalsamy, P. (2009). Taguchi experimental design for medium optimization for enhanced protease production by Bacillus subtilis HB04. "e-Journal of Science & Technology". [Online] 4(4): 1-10. Available from: http://e-jst.teiath.gr/
Journal: e-Περιοδικό Επιστήμης & Τεχνολογίας
e-Journal of Science & Technology
Type of Journal: With a review process (peer review)
Access scheme: Publicly accessible
License: Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες
URI: http://hdl.handle.net/11400/4995
Appears in Collections:Τόμος 04, τεύχος 4 (2009)

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