Please use this identifier to cite or link to this item: http://hdl.handle.net/11400/5539
Title: Πολυφασική μελετη ταυτοποίησης, τυποποίησης και προσδιορισμού ευαισθησίας στελεχών για προσδιορισμό δεικτών λοιμογονικότητας και ανάπτυξη εργαστηριακών διαδικασιών κλινικής εφαρμογής
Clinical-oriented polyphasic identification, typing and susceptibility methodology for Candida rugosa isolates to establish potential virulence markers and processing guidelines
Authors: Κόλλια, Κ.
Καμπούρης, Μανούσος Ε.
Μπελάζη, Μαρία
Κουσσίδου-Ερεμόνδη, Τ.
Ανδρεάδης, Δ.
Contributors: Βελεγράκη, Αριστέα
Item type: Conference publication
Keywords: PCR-RFLP;Bloodstream fungal infections;Candida rugosa;Μυκητιασικές λοιμώξεις κυκλοφορίας αίματος;TTC culture;TTC πολιτισμός;Electrophoretic karyotyping;Ηλεκτροφορητικό καρυότυπο
Subjects: Science
Microbiology
Επιστήμες
Μικροβιολογία
Issue Date: 3-Feb-2015
2012
Date of availability: 3-Feb-2015
Publisher: Νερατζής, Ηλίας
Σιανούδης, Ιωάννης
Abstract: Candida rugosa is a rare as yet, though emerging pathogen, attracting increasing international interest. A collection of 10 clinical strains from the southern Balkan peninsula was subjected to polyphasic detection, identification and typing techniques, such as cultures in differential media, microscopy, assimilation tests, immunefluorescence, in vitro drug susceptibility (microdilution CLSI methodology and E-test) to multiple agents and both simple and amplification-based molecular techniques, such as electrophoretic karyotyping, RFLP-PFGE, PCR fingerprinting with mini- and microsatellite DNA, ribotyping and multiple PCR-RFLPs. Similarities and differences to other members of the genus were noted to determine appropriate identification, typing and treatment techniques and regimens for use in epidemiological studies and clinical applications, as the resulting C. rugosa profiles were in different degrees distinct but generally not dissimilar to other Candida species. TTC assimilation and Dalmau cultures allow identification, but typing is better achieved with electrophoretic karyotyping and specific PCR-RFLP, though the respective results are similar but not matching, indicating discontinuous intraspecific microevolutionary events.
Language: English
Citation: Kollia, K., Kambouris, M.E., Belazi, M., Koussidou-Eremondi, T., Andreadis, D., et al. (2012). Clinical-oriented polyphasic identification, typing and susceptibility methodology for Candida rugosa isolates to establish potential virulence markers and processing guidelines. "e-Journal of Science & Technology". [Online] 7(1): 43-54. Available from: http://e-jst.teiath.gr/
Journal: e-Περιοδικό Επιστήμης & Τεχνολογίας
e-Journal of Science & Technology
Type of Journal: With a review process (peer review)
Access scheme: Publicly accessible
License: Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες
URI: http://hdl.handle.net/11400/5539
Appears in Collections:Τόμος 07, τεύχος 1 (2012)

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