Please use this identifier to cite or link to this item: http://hdl.handle.net/11400/9851
Title: Σημασία των integrons και πολλαπλής αντοχής σε στελέχη εντεροβακτηριακών από υγιή και νοσούντα κοτόπουλα πτηνοτροφείων
Integrons and multidrug resistance among Enterobacteriaceae from sick and healthy chickens from the Greek poultry enterprise
Authors: Πόγγας, Νικόλαος Γ. (1949- )
Δάϊκος, Γεώργιος, Κ.
Βασιλακοπούλου, Α.
Πασιώτου - Γαβαλά, Μαρία
Γαζούλη, Μαρία
Contributors: Χάρβαλου, Αικατερίνη
Πετρίκκος, Γεώργιος, Λ.
Item type: Journal article
Keywords: Laboratory research;Avian influenza;Chicken bacteria;Antibiotic resistance;Εργαστηριακή έρευνα;Γρίπη των πουλερικών;Βακτήρια κοτόπουλων;Αντιβιοτική αντοχή
Subjects: Medicine
Microbiology
Ιατρική
Μικροβιολογία
Issue Date: 7-May-2015
2007
Date of availability: 7-May-2015
Language: Greek
Citation: Πόγγας, Ν., Δάϊκος, Γ., Βασιλακοπούλου, Α., Πασιώτου, Μ., Γαζούλη, Μ., κ.ά. (2007) Σημασία των integrons και πολλαπλής αντοχής σε στελέχη εντεροβακτηριακών από υγιή και νοσούντα κοτόπουλα πτηνοτροφείων. "Δελτίο Ελληνικής Μικροβιολογικής Εταιρείας", 52 (1), σ. 42-46. Διαθέσιμο στο: http://hms.org.gr/updocuments/Book2007-1.pdf [Έγινε Πρόσβαση: 07/05/2015].
Journal: Δελτίο Ελληνικής Μικροβιολογικής Εταιρείας
Type of Journal: With a review process (peer review)
Table of contents: Objective: The purpose of this study was to evaluate multidrug-resistance (MDR) and preva-lence of class I, II, III integrons in avian Enterobacteriaceae from Greek poultry enterprises. Material-Methods: Forty-four avian MDR (resistant to more than 2 antibiotics) Enterobac-teriaceae strains were isolated from sick and healthy chickens and identified by the API 20E system. Antibiotic sensitivity testing was performed by the Kirby-Bauer method. Degenerated primers were used to amplify the integrase I, II and III genes. The PCR products were analyzed by RFLP, following RsaI digestion. Amplification by PCR of the integron class I and II cassette was performed and the products were sequenced. Phylogeny study was performed by RAPD (random amplified polymorphic DNA analysis). Results: Twenty-three strains contained inte-gron class I, five contained integron class II and six strains contained both class I and class II. Ten MDR isolates from sick poultry harboured integrons. Integron-positive isolates were also more likely to be multi-resistant than integron-negative isolates. The acquisition of resistance genes seems not to be random, and the transfer of integron-carrying elements plays a dominant role in the development of MDR by Enterobacteriaceae. Conclusions: Integrons are important vehicles for the transfer of MDR to the environment and the food chain and represent a threat to the poultry industry. It is clear that in the poultry enterprise environment the dissemination of MDR is promoted strongly via a horizontal transfer. However, the number of multidrug re-sistant strains without integron shows that other factors are also associated with the spread of antibiotic resistance in poultry. Elucidation of the parameters of this phenomenon and the possible pathways of transfer to humans via the food chain is an important task which needs to be accomplished.
Access scheme: Publicly accessible
License: Αναφορά Δημιουργού-Μη Εμπορική Χρήση-Όχι Παράγωγα Έργα 3.0 Ηνωμένες Πολιτείες
URI: http://hdl.handle.net/11400/9851
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